sbcB15 and Δ sbcB Mutations Activate Two Types of RecF Recombination Pathways in Escherichia coli
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چکیده
منابع مشابه
Escherichia coli xonA (sbcB) mutants enhance illegitimate recombination.
Mutations of Escherichia coli K-12 were isolated that increase the frequency of deletion formation. Three of these mutations map to the gene sbcB at 43.5 min on the E. coli chromosome. Two types of mutations at sbcB have been previously defined: sbcB-type that suppress both the UV sensitivity and recombination deficiency of recBC mutants, and xonA-type that suppress only the UV sensitivity. Bot...
متن کاملEvidence that SbcB and RecF pathway functions contribute to RecBCD-dependent transductional recombination.
A role for the RecF, RecJ, and SbcB proteins in the RecBCD-dependent recombination pathway is suggested on the basis of the effect of null recF, recJ, and sbcB mutations in Salmonella typhimurium on a "short-homology" P22 transduction assay. The assay requires recombination within short (approximately 3-kb) sequences that flank the selected marker and lie at the ends of the transduced fragment....
متن کاملHomologous Recombination by the RecBCD and RecF Pathways
In all cells, genetic recombination is used to repair DNA breaks and, as a result, genetic information is exchanged between homologous DNA molecules. Discontinuities in DNA strands, specifically double-strand DNA breaks and single-strand DNA gaps, attract the enzymes responsible for the initiation of homologous recombination. In wild-type Escherichia coli, two distinct pathways are responsible ...
متن کاملGenetic and physical analysis of plasmid recombination in recB recC sbcB and recB recC sbcA Escherichia coli K-12 mutants.
The effect of mutations in known recombination genes (recA, recB, recC, recE, recF, recJ, recN, recO, recQ and ruv) on intramolecular recombination of plasmids was studied in recB recC sbcB and recB recC sbcA Escherichia coli mutants. The rate of recombination of circular dimer plasmids was at least 1000-fold higher in recB recC sbcB or recB recC sbcA mutants as compared to wild-type cells. The...
متن کاملPhage X Has an Analog of Escherichia coli rec 0 , recR and recF Genes
The RecF pathway catalyzes generalized recombination in Escherichia coli that is mutant for recBC, sbcB and sbcC. This pathway operating on conjugational recombination requires the recA, recF, recJ, recN, rec0, recQ, recR, ruvA, ruvB and ruvC genes. In contrast, X mutant for its own recombination genes, int, reda and red& requires only the recA and reg genes to recombine efficiently in recBC sb...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2006
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00613-06